产品货号：
Z15122
中文名称：
DDX4抗体
英文名称：
Anti-DDX4 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-DDX4 Antibody |
| 指标别名 | DDX4;DDX4;VASA;DEAD box protein 4;VASA;Vasa homolog |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,IF,ICC/IF,FCM,ELISA |
| 基因名称 | DDX4 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human DDX4/MVH recombinant protein (Position:D3-D666). |
| 实际分子量 | 79KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | DDX4(DEAD/H BOX 4),also known as VASA.The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins.The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins.By radiation hybrid analysis,Castrillon et al.(2000) mapped the VASA gene to 5q.By fluorescence in situ hybridization,they refined the localization to 5q11.2-q12.This region is syntenic to the distal end of mouse chromosome 13,where the mouse VASA homolog(Ddx4) resides(Abe and Noce,1997).Using a combination of proteomics,cytology,and functional analysis in C.elegans,Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis. |
| Uniprot ID | Q9NQI0 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells (ELISA)： 1:100～1000 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Immunofluorescence (IF) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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